Colonially oriented historians have asserted that the Maya had left the area long before the arrival of British settlers. But many Maya were still in Belize when the Europeans came in the sixteenth and seventeenth centuries. Archaeological and ethnohistorical research confirms that several groups of Mayan peoples lived in the area now known as Belize in the sixteenth century. The political geography of that period does not coincide with present-day boundaries, so several Mayan provinces lay across the frontiers of modern Belize, Mexico, and Guatemala. The Mayan province of Chetumal, for example, consisted of the northern part of presentday Belize and the southern coast of the Mexican state Quintana Roo. In the south, spreading west over the present-day frontier between Belize and Guatemala, were the Mopán Maya, and still farther south, the Chol-speaking Manche groups. In central Belize lay the province of Dzuluinicob, meaning "land of foreigners" or "foreign people." This province stretched from New River in the north to Sittee River in the south, and from close to the presentday Guatemalan border in the west to the sea. The apparent political center of this province was Tipu, located east of modern Benque Viejo del Carmen. Lamanai, several towns on New River and on Belize River, and Xibún on Sibun River, were included in this province.

Christopher Columbus traveled to the Gulf of Honduras during his fourth voyage in 1502. A few years later, two of his navigators, Martín Pinzón and Juan De Solís, sailed northward along the coast of Belize to Yucatán. In 1519 Hernán Cortés conquered Mexico, and Pedro Arias Dávila founded Panama City. Spain soon sent expeditions to Guatemala and Honduras, and the conquest of Yucatán began in 1527. When Cortés passed through the southwestern corner of present-day Belize in 1525, there were settlements of Cholspeaking Manche in that area. When the Spanish "pacified" the region in the seventeenth century, they forcibly displaced these settlements to the Guatemalan highlands. The Spanish launched their main incursions into the area from Yucatán, however, and encountered stiff resistance from the Mayan provinces of Chetumal and Dzuluinicob. The region became a place of refuge from the Spanish invasion, but the escaping Maya brought with them diseases that they had contracted from the Spanish. Subsequent epidemics of smallpox and yellow fever, along with endemic malaria, devastated the indigenous population and weakened its ability to resist conquest.

In the seventeenth century, Spanish missionaries from Yucatán traveled up New River and established churches in Mayan settlements with the intention of converting and controlling these people. One such settlement was Tipu, which was excavated in the 1980s. People occupied the site during preclassic, classic, and postclassic times, and through the conquest period until 1707. Though conquered by the Spanish in 1544, Tipu was too far from the colonial centers of power to be effectively controlled for long. Thousands of Maya fled south from Yucatán in the second half of the sixteenth century, and the people of Tipu rebelled against Spanish authority. Although Tipu was too far south for the Spanish of Yucatán to control, it was apparently too important to ignore because of its proximity to the Itzá of the Lago Petén Itzá region of present-day Guatemala. In 1618 and 1619, two Franciscans, attempting to convert the people built a church in Tipu. In 1638 a period of resistance began in Tipu, and by 1642, the entire province of Dzuluinicob was in a state of rebellion. The Maya abandoned eight towns at this time, and some 300 families relocated in Tipu, the center of rebellion. In the 1640s, Tipu's population totaled more than 1,000.

Piracy along the coast increased during this period. In 1642, and again in 1648, pirates sacked Salamanca de Bacalar, the seat of Spanish government in southern Yucatán. The abandonment of Bacalar ended Spanish control over the Mayan provinces of Chetumal and Dzuluinicob.

Between 1638 and 1695, the Maya living in the area of Tipu enjoyed autonomy from Spanish rule. But in 1696, Spanish soldiers used Tipu as a base from which they pacified the area and supported missionary activities. In 1697 the Spanish conquered the Itzá, and in 1707, the Spanish forcibly resettled the inhabitants of Tipu to the area near Lago Petén Itzá. The political center of the Mayan province of Dzuluinicob ceased to exist at the time that British colonists were becoming increasingly interested in settling the area.

Data as of January 1992